Experimental Soft Condensed Matter Group Professor David A. Weitz Division of Engineering and Applied Sciences Department of Physics Harvard University
NIR excited fluorophores for biomedical applications
We are interested on optimizing the fluorescence of near infrarred excited fluorophores for biomedical applications. In particular, we characterized the fluorescence of upconverting nanoparticles (UCNPs) when they are in close proximity to metal nanoparticles (MNPs), quantum dots (QDs) or graphene oxide for modifying their emission and excitation properties. We use this probes for biosensing. We also optimized NIR probes as Ag2S for in vivo fluorescence detection in the second biological window. We experimentally characterize their quantum yield, fluorescence lifetimes and fluorescence spectra.
  • Plasmonic effects in hybrid systemsPlasmon-enhanced UCNPs fluorescence using metal nanoparticles; Plasmonic effects in QDs-MNPs hybrid systems
  • Sensors based on UCNPs fluorescence: for miRNA detection; FRET from UCNPs to QDs for biosensors  
  • Determination of UCNPs and NIR probes fluorescence lifetimes and quantum yield measurements
KEYWORDS: Upconverting nanoparticles (UCNPs), Quantum dots (QDs), Metal nanoparticles (MNPs), Forster resonance energy transfer (FRET), Fluorescence lifetime, quantum yield (QY)

Fluorescence_Microscopy  Quantum-Yield2
Experimental setup built to determine luminescence spectra, quantum yield and fluorescence lifetimeof UCNPs
Er Yb energy levelsCryostat

We experimentally analyzed the enhancement of the upconversion fluorescence emission intensity at cryogenic temperatures.
Egatz-Gómez et al. J. Lumin. 139, 52 (2013) [PDF]
  

 
miRNA_detection_assaymiRNA sensor -FRET UCNPs  
We collaborate with MATNABIO Reseach Group led by Jorge Rubio-Retama from the Faculty of Pharmacy at UCM within different projects (PR26/16-12B, MAT2017-83111R) regarding miRNA biosensors based on FRET processes in UCNPs.
We use UCNPs as molecular beacons to detect extremely low concentrations of target (100fM). 
Méndez-González et al. ACS Appl. Mater. Interfaces 9, 12272 (2017) [PDF]
We explore different strategies to improve FRET efficiency in UCNPs-QDs hybrid systems. Melle et al. J. Phys. Chem. C 122, 18751-18758 (2018) [PDF]
FRET_UCNPs-QDs
QD-MNP
We theoretically studied the THz emission in the fluorescence spectrum of a QD coupled to an spherical metallic nanoparticle Antón et al. J. Opt. 18, 025001 (2016) [PDF]
 
AuNRs-UCNPs
We studied plasmon-enhanced UCNPs fluorescence using polyelectrolyte multilayers as spacers between UCNPs and Au nanorods



 
Research Group:

Oscar G. Calderón, Departamento Óptica, UCM
Eduardo Cabrera-Granado,
Departamento Óptica, UCM
Miguel Antón Revilla, Departamento Óptica, UCM
Fernando Carreño, Departamento Óptica, UCM

Collaborators 
Ana Egatz Gómez, School of Molecular Science, Arizona State University
Jorge Rubio Retama, MATNABIO, Departamento Química en CC. Farmacéuticas, UCM
Elena Díaz, GISC, Departamento Materiales, UCM


Publications

International meetings

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